[Chemical constituents from Daphne acutiloba].

To study the chemical constituents from Daphne acutiloba. The constituents were separated by column chromatography, and their structures were elucidated by spectroscopic data analyses. Fifteen compounds were isolated from the EtOAc extract and identified as wikstroelide M (1), vesiculosin (2), prostratin (3), 7-hydroxy-coumarin (4), 7,8-di-hydroxy-coumarin (5), isodaphnoside (6), daphnorine (7), rutamontine (8), daphnolin (9), daphneticin (10), (+)-pinoresinol-beta-D-glucoside (11), oleodapnone (12), oleodaphnal (13), ergosterol peroxide (14) and cholest-5-en-3beta-ol (15). All the compounds except for 4, 5 and 14 were obtained from the stems of this plant for the first time.