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  • Membrane filtration of Sudan orange G on a cellulose acetate membrane filter for separation-preconcentration and spectrophotometric determination in water, chili powder, chili sauce and tomato sauce samples.

Membrane filtration of Sudan orange G on a cellulose acetate membrane filter for separation-preconcentration and spectrophotometric determination in water, chili powder, chili sauce and tomato sauce samples.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (2012-05-24)
Zeid A ALOthman, Yunus E Unsal, Mohamed Habila, Azza Shabaka, Mustafa Tuzen, Mustafa Soylak
ABSTRACT

A simple membrane filtration procedure for separation-enrichment of Sudan orange G is presented. The method is based on the adsorption of Sudan orange G on a cellulose acetate filter and its elution from the membrane with 10 mL of ethanol. Sudan orange G in the eluent was determined by UV-visible spectrophotometry at 388 nm. The effect of analytical conditions, including pH, flow rates and eluent, sample volume, type of membrane for quantitative preconcentration and separation of Sudan orange G were examined. The influences of matrix components on Sudan orange G recoveries were studied. The preconcentration factor was 125. The detection limit was 4.9 μg L(-1). The relative standard deviation was 4.3%. The presented procedure was applied to chili powder, chili sauce, tomato sauce, powdered beverage and water samples.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Cellulose acetate, average Mn ~30,000 by GPC
Sigma-Aldrich
Cellulose acetate, average Mn ~50,000 by GPC