Detection of 500-nucleotide DNA by laser desorption mass spectrometry.

Detection of 500-nucleotide DNA by laser desorption mass spectrometry.

Rapid communications in mass spectrometry : RCM (1994-09-01)

K Tang, N I Taranenko, S L Allman, L Y Cháng, C H Chen

ABSTRACT

We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.

MATERIALS

Product Number

Brand

Product Description

152307

Sigma-Aldrich

3-Hydroxypyridine-2-carboxylic acid, 98%

56197

Supelco

3-Hydroxypicolinic acid, matrix substance for MALDI-MS, ≥99.0% (HPLC)