ZRB1287
Anti-CD11c Antibody, clone 1D6 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1D6, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 127.38 kDa
observed mol wt ~130 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1D6 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects CD11c. It targets an epitope within 15 amino acids form the N-terminal, extracellular domain.
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal, extracellular domain of human Integrin alpha-X (CD11c).
Application
Quality Control Testing
Evaluated by Western Blotting in THP-1 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD11c in THP-1 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD11c in U937 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD11c in THP-1 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD11c in human tonsil tissue sections.
Flow Cytometry Analysis: 1 μg from a representative lot detected CD11c in one million THP-1 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in THP-1 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD11c in THP-1 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD11c in U937 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD11c in THP-1 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD11c in human tonsil tissue sections.
Flow Cytometry Analysis: 1 μg from a representative lot detected CD11c in one million THP-1 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CD11c, clone 1D6 ZooMAb®, Cat. No. ZRB1287, is a recombinant Rabbit monoclonal antibody that specifically targets CD11c and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Integrin alpha-X (UniProt: P20702; also known as CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150-95, CD11c) is encoded by the ITGAX (also known as CD11C) gene (Gene ID: 3687) in human. CD11c is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 20-1107), a transmembrane domain (aa 1108-1128), and a cytoplasmic domain (aa 1129-1163). CD11c is predominantly expressed in macrophages, monocytes, and granulocytes and along with integrin b2 it serves as a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. The aXb2 heterodimer binds many ligands, including collagen type 1, iC3b, fibrinogen, ICAM-1, and lipopolysaccharide. It is reported to mediate cell-cell interaction during inflammatory responses and is especially important in monocyte adhesion and chemotaxis. The I domains of CD11 are responsible for the binding of ligands and have a unique structure with 6-7 a -helixes and 6 b sheets with interconnecting loops. In CD11c the loops on the I domain are involved in fibrinogen binding and loops a3- a4 and bD-a5 are reported to be more important for recognition of fibrinogen. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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