P7937

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit

5,000-15,000 units/mg protein, recombinant, expressed in E. coli, lyophilized powder

• Synonym(s): Protein phosphatase
• Enzyme Commission number: 3.1.3.16 (BRENDA, IUBMB)
• MDL number: MFCD01635658
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥80% (GE)
• form: lyophilized powder
• specific activity: 5,000-15,000 units/mg protein
• mol wt: 37.5 kDa
• technique(s): inhibition assay: suitable
• UniProt accession no.: P62139
• storage temp.: −20°C
• Gene Information: rabbit ... PPP1CA(100009298)

Description

General description

Protein phosphatase1 (PP1) is a heterodimeric enzyme with serine/threonine phosphatase activity. It comprises a catalytic subunit and a targeting subunit or a specific protein inhibitor. The targeting subunit determines the enzyme localization, e.g., to glycogen particles (subunit G) or to myofibrils (subunit M).

Application

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used as a standard in protein phosphatase-1 inhibition assay.

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used in protein phosphatase reaction and in the digestion of adipocyte extracts.

Biochem/physiol Actions

Protein phosphatase1 (PP1) is involved in glycogen metabolism and in regulation of muscle contractility. In addition, it is implicated in cell cycle and transcriptional regulation. Recombinant rabbit PP1α has properties in common with the native rabbit muscle protein including size, requirement for Mn²⁺ for activity, specificity for phosphorylase A, and inhibition by okadaic acid, microcystin LR, and phosphatase inhibitor2 (I-2).

Packaging

Package size based on protein content.

Unit Definition

One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per min at pH 7.4 at 30 °C.

Physical form

Lyophilized powder containing imidazole buffer, pH 7.4, NaCl, DTT, EDTA, MnCl₂, Tween 20, and trehalose as stabilizer.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves