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D2004

Sigma-Aldrich

4-(Dimethylamino)benzaldehyde

suitable for histochemical demonstration of nitro blue tetrazolium reduction in neutrophils

Synonym(s):

p-Dimethylaminobenzaldehyde, DMAB, Ehrlich’s reagent

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About This Item

Linear Formula:
(CH3)2NC6H4CHO
CAS Number:
100-10-7
Molecular Weight:
149.19
Beilstein:
606802
EC Number:
202-819-0
MDL number:
MFCD00003381
UNSPSC Code:
12171500
PubChem Substance ID:
57654078
NACRES:
NA.47

Assay

≥99% (TLC)

form

powder

mp

72-75 °C (lit.)

solubility

ethanol: 50 mg/mL

suitability

suitable for histochemical demonstration of nitro blue tetrazolium reduction in neutrophils

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc(C=O)cc1

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

InChI key

BGNGWHSBYQYVRX-UHFFFAOYSA-N

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Application

4-(Dimethylamino)benzaldehyde (DMAB or Ehrlich’s reagent) has been used as a color reagent for the determination of hydroxyproline level.It forms colored condensation products (Schiff bases) with pyrroles and primary amines. DMAB is also suitable as a reagent to develop latent fingermarks on paper surfaces yielding impressions that are both colored and photoluminescent.
Forms colored condensation products (Schiff bases) with pyrroles and primary amines.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1B

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

327.2 °F - closed cup

Flash Point(C)

164 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Jamie Morrison et al.
The American journal of pathology, 166(6), 1701-1710 (2005-05-28)
Duchenne muscular dystrophy was initially described as a myosclerosis because of the conspicuous progression of interstitial fibrosis. Using the mdx mouse homologue, we have shown previously that the accumulation of intramuscular collagen is profoundly influenced by the presence or absence
Jun-Min Guo et al.
Journal of agricultural and food chemistry, 58(11), 6556-6561 (2010-05-15)
A simple colorimetric method for the differentiation of indoleacetic acid (IAA) and indolebutyric acid (IBA) in plant samples is described. The color change is based upon the reaction between the auxins and p-(dimethylamino)benzaldehyde (PDAB, Ehrlich reagent) following the electrophilic substitution
Andrew P Breksa et al.
Journal of agricultural and food chemistry, 55(13), 5013-5017 (2007-06-05)
A method for estimating the total limonoid aglycone and glucoside concentrations in Citrus samples in terms of limonin and limonin glucoside equivalents is presented. The method consists of extraction followed by colorimetric quantification. The colorimetric quantification was based on the
J D Brady et al.
The Journal of biological chemistry, 276(22), 18812-18818 (2001-03-30)
The structures of pyrrolic forms of cross-links in collagen have been confirmed by reacting collagen peptides with a biotinylated Ehrlich's reagent. This reagent was synthesized by converting the cyano group of N-methyl-N-cyanoethyl-4-aminobenzaldehyde to a carboxylic acid, followed by conjugation with
F J Hidalgo et al.
Analytical biochemistry, 262(2), 129-136 (1998-09-29)
The Ehrlich reaction was optimized to determine pyrrolized proteins produced as a consequence of lipid peroxidation and oxidative stress. The procedure consisted of the treatment of the modified protein with p-(dimethylamino)benzaldehyde at a controlled acidity and temperature, and the determination
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