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17170

Millipore

AEA Sporulation Broth (Base), modified

NutriSelect® Plus, suitable for microbiology

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

ammonium acetate, 1.5 g/L
biopeptone, 10 g/L
disodium phosphate, 4.36 g/L
magnesium sulfate.7H2O, 0.2 g/L
monopotassium phosphate, 0.25 g/L
yeast extract, 10 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.8±0.2 (25 °C)

application(s)

food and beverages
microbiology

suitability

nonselective for Clostridium spp.

Related Categories

Application

For early sporulation of Clostridium perfringens from foods.

Preparation Note

Suspend 26.31 g in 1 litre distilled water. Dispense the medium in 15 ml amounts in screw capped tubes and sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and add 0.6 ml of filter sterilized 10% raffinose and 0.2 ml each of sterile 0.66 M sodium carbonate and 0.32% cobalt chloride dropwise to each 15 ml base medium in the tubes. Just before use, steam the medium for 10 minutes and after cooling, add 0.2 ml of filter sterilized (freshly prepared) 1.5% sodium ascorbate to each tube of the medium.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCC7226
BCBW2628
BCBT5793
BCBM5864V
BCBK7985V

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Joyshree Ganguly et al.
Anaerobe, 63, 102208-102208 (2020-05-11)
Single-cell analysis of microbial population heterogeneity is a fast growing research area in microbiology due to its potential to identify and quantify the impact of subpopulations on microbial performance in, for example, industrial biotechnology, environmental biology, and pathogenesis. Although several

Articles

Clostridia Diagnostic

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.

Clostridium perfringens, their Properties and their Detection

An article regarding the Role of Clostridium perfringens and their detection, identification, and differentiation from Sigma-Aldrich.com

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