219402

Cathepsin L, Human Liver

Cathepsin L, Human Liver, CAS 60616-82-2, is a native, the most potent of all the lysosomal proteinases. Plays a major role in the proteolysis of both cellular and endocytosed macromolecules.

• Enzyme Commission number: 3.4.22.15 (BRENDA, IUBMB)
• UNSPSC Code: 12352202

Properties

• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Cathepsin L, Human Liver, CAS 60616-82-2, is a native, the most potent of all the lysosomal proteinases. Plays a major role in the proteolysis of both cellular and endocytosed macromolecules.

Native cathepsin L from human liver. The most potent of the lysosomal proteinases, having a higher activity than cathepsins B and H in the degradation of a variety of physiological protein substrates. Cathepsin L is believed to be responsible for the generation of endostatin from NC1 domain in collagen XVII.

Native cathepsin L from human liver. The most powerful of the lysosomal proteinases, having a higher specific activity than cathepsins B and H in the degradation of a variety of physiological protein substances.

Note: 1 mU = 1 milliunit.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Z-FR-AFC per min at 25°C, pH 5.5.

Physical form

In 400 mM NaCl, 20 mM malonate buffer, 1 mM EDTA, pH 5.5.

Preparation Note

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Kurata, M., et al. 2001. J. Biochem. (Tokyo)130, 857.
Ferreras, M., et al. 2000. FEBS Lett.486, 247.
Baricos, W.H., et al. 1988. Biochem. J.252, 301.
McDonald, J.K., et al. 1988. Biochem. Biophys. Res. Commun.151, 827.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable