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HPLC Analysis of Proteins on BIOshell A160 Peptide C18

HPLC Analysis of Proteins on BIOshell A160 Peptide C18 application for HPLC

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

53307-U

Colonna HPLC Ascentis® peptidi Express ES- C18, 2,7 μm

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

Standard

N° Catalogo
Descrizione
Determinazione del prezzo

T9128

Inibitore della tripsina

lyophilized powder

R6513

Ribonucleasi A

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

62971

Lisozima

powder (crystalline), ~70000 U/mg

S7571

Superoxide Dismutase from bovine erythrocytes

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

C6749

Cytochrome c equine

recombinant, expressed in E. coli

C2624

Anidrasi carbonica

≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder

CONDITIONS

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile

gradient

held at 0% B for 0.5 min; 0 to 100% B in 7 min

flow rate

0.3 mL/min

pressure

4743 psi (327 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

1 μL

sample

0.2 - 0.6 g/L in mobile phase A

Descrizione

Note legali

null

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Related Applications

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