- Metabolic profiling of urinary organic acids by single and multicolumn capillary gas chromatography.
Metabolic profiling of urinary organic acids by single and multicolumn capillary gas chromatography.
Journal of chromatographic science (1989-01-01)
M F Lefevere, B J Verhaeghe, D H Declerck, J F Van Bocxlaer, A P De Leenheer, R M De Sagher
PMID2925825
ABSTRACT
High-resolution gas chromatography (HRGC) and gas chromatography/mass spectrometry (GC/MS) are the techniques of choice to determine the retention indices of more than 200 organic acids as their trimethylsilyl (TMS) or oxime-trimethylsilyl derivatives. Several types of apolar and semipolar fused-silica capillary columns (OV-1, SE-52, and OV-1701), used to analyze and separate organic acids isolated from urine samples, are evaluated.
MATERIALI