T5076

Anti-β-Tubulin Isotype III antibody, Mouse monoclonal

clone SDL.3D10, purified from hybridoma cell culture

• Sinonimo/i: Anti-CDCBM, Anti-CDCBM1, Anti-CFEOM3, Anti-CFEOM3A, Anti-FEOM3, Anti-TUBB4, Anti-beta-4
• Numero MDL: MFCD08705576
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: SDL.3D10, monoclonal
• Forma fisica: buffered aqueous solution
• Reattività contro le specie: mouse, rat, human, boar, bovine
• Confezionamento: antibody small pack of 25 μL
• Concentrazione: ~2 mg/mL
• tecniche: dot blot: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.1-0.2 μg/mL using rat brain extract
• Isotipo: IgG2b
• N° accesso UniProt: Q13509
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TUBB3(10381) ; mouse ... Tubb3(22152) ; rat ... Tubb3(246118)

Descrizione

Descrizione generale

Tubulin β 3 class III (TUBB3) encodes for the microtubule protein tubulin β‐3, which is majorly expressed in neurons. The gene is located on human chromosome 16q24.3.

Tubulin is the main constituent of microtubules made up of two similar polypeptides α and β, each with 50 kD (approx.) molecular weight. It has several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin isotype III antibody can be used in microarray and western blotting. It can also be used in immunoblotting. Mouse anti-β-tubulin isotype III antibody reacts specifically with an epitope present on the β-tubulin (isotype III) of bovine, rat, mouse, boar and human but not with tubulin present in chicken brain preparation or in a chicken fibroblasts cell line.

Immunogeno

peptide corresponding to the carboxy-terminal sequence of human β-tubulin isotype III, conjugated to BSA.

Applicazioni

Monoclonal Anti-β-Tubulin Isotype III antibody produced in mouse has been used in immunolabelling and flow cytometry.

Monoclonal anti-β-tubulin isotype III antibody (diluted 1:500) can be used as a primary antibody in immunocytochemical analysis of neurons. It can also be used for the localization of isotype III of β-tubulin by several immunochemical assays like dot blot, ELISA and immunohistochemistry.

Azioni biochim/fisiol

Tubulin β 3 class III (TUBB3) regulates neurogenesis and is involved in axon guidance and maintenance. Mutations in this gene is associated with hypoplasia of commissural axon (CA), congenital fibrosis of the extraocular muscles type 3 (CFEOM3) and dysgyria.

Stato fisico

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable