SAB4200847

Anti-FAM134B antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: JK1, RETREG1, reticulophagy regulator 1
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Forma dell’anticorpo: affinity isolated antibody
• Livello qualitativo: 200
• Reattività contro le specie: human
• Concentrazione: ~1 mg/mL
• tecniche: indirect immunofluorescence: 1:500-1:1000 using cultured human Hela Cells; western blot: 1:500-1:1000 using 293T Cells Exp.FAM134B
• N° accesso UniProt: Q9H6L5
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Five endoplasmic reticulum (ER)-resident proteins have been shown to function as receptors for selective ER-phagy: FAM134B, SEC62, RTN3, CCPG1 and ATL3.1-2 These integral membrane proteins appear to partition into different sub-domains of the ER. All of them harbor a sequential peptide motif within the cytoplasmic region, enabling binding to LC3/GABARAP proteins associated with phagophore membrane.1 FAM134B (also known as RETREG1 or JK-1) is the first ER-phagy receptor to be identified.2

Specificità

Anti-FAM134B antibody specifically recognizes an epitope on human FAM134B cytoplasmic domain.

Applicazioni

The antibody may be used in various immunochemical techniques including Immunoblotting (may appear as two isoforms ~55 kDa and ~40 kDa) and Immunofluorescence. Detection of the FAM134B band by Immunoblotting is specifically inhibited by the immunogen.

Azioni biochim/fisiol

The protein was discovered to be involved in the pathogenesis of esophageal squamous cell carcinoma (ESCC).3-4 Loss of function mutations in FAM134B result in severe sensory and autonomic neuropathy (HSANII).5 In addition, FAM134B has been classified as an intra-membrane ER-resident protein that is characterized by the presence of a reticulon homology domain (RHD) and that is mainly located at the edges of the ER sheets.1,6 In research, FAM134B is implicated in the suppression of viral replication during Ebola, Dengue, Zika and West Nile viral infections.7-8 Non-structural viral proteases such as NS2S3 specifically cleave FAM134B, thereby subverting ER-phagy.7 Specific mutations in FAM134B and expression profiles are strongly correlated with colorectal cancers.9 Increased expression of FAM134B is also implicated with susceptibility to vascular dementia and allergic rhinitis.9-10

Stato fisico

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Stoccaggio e stabilità

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable