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P4716

Sigma-Aldrich

Pectinase from Aspergillus niger

greener alternative

BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

Sinonimo/i:

Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger

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About This Item

Numero CAS:
9032-75-1
Classificazione EC (Enzyme Commission):
3.2.1.15 (BRENDA, IUBMB)
Numero CE:
232-885-6
Numero MDL:
MFCD00131809
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

Aspergillus niger

Livello qualitativo

200

Nome Commerciale

BioReagent

Forma fisica

aqueous glycerol solution

Attività specifica

≥5 units/mg protein (Lowry)

Caratteristiche più verdi

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

tecniche

cell culture | plant: suitable

Categoria alternativa più verde

, Enabling

Temperatura di conservazione

2-8°C

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Descrizione generale

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Applicazioni

Petctinase from Aspergillus niger has been used:
  • for protoplasts liberation
  • in the protoplast enzyme mixture for enzyme digestion
  • to conduct partial saccharification of sugars
  • to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Azioni biochim/fisiol

Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans.

Definizione di unità

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

Stato fisico

Solution in 40% glycerol

Altre note

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Karyotypes of some species of Castanopsis, Lithocarpus and Quercus (Fagaceae) from Khun Mae Kuang Forest in Chiang Mai province, northern Thailand
Chokchaichamnankit P, et al.
Thai Forest Bulletin , 38-44 (2007)
Tony Heitkam et al.
The Plant journal : for cell and molecular biology, 103(1), 32-52 (2020-01-26)
If two related plant species hybridize, their genomes may be combined and duplicated within a single nucleus, thereby forming an allotetraploid. How the emerging plant balances two co-evolved genomes is still a matter of ongoing research. Here, we focus on
Tolerance mechanisms in mercury-exposed Chromolaena odorata (lf) RM King et H. Robinson, a potential phytoremediator
Alcantara HJP, et al.
Journal of Degraded and Mining Lands Management, 1(1), 09-20 (2013)
Trude Schwarzacher
Methods in molecular biology (Clifton, N.J.), 1370, 87-103 (2015-12-15)
Good preparations are essential for informative analysis of both somatic and meiotic chromosomes, cytogenetics, and cell divisions. Fluorescent chromosome staining allows even small chromosomes to be visualized and counted, showing their morphology. Aneuploidies and polyploidies can be established for species
Paulina Tomaszewska et al.
Frontiers in plant science, 13, 1026364-1026364 (2022-12-10)
Structural chromosome rearrangements involving translocations, fusions and fissions lead to evolutionary variation between species and potentially reproductive isolation and variation in gene expression. While the wheats (Triticeae, Poaceae) and oats (Aveneae) all maintain a basic chromosome number of x=7, genomes
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Articoli

Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Protocolli

Enzymatic Assay of Pectinase

Enzymatic Assay of Pectinase

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.