N2280

Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse

clone NOS-B1, ascites fluid

• Sinonimo/i: Anti-bNOS
• Numero MDL: MFCD01092550
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: ascites fluid
• Tipo di anticorpo: primary antibodies
• Clone: NOS-B1, monoclonal
• PM: antigen 150-160 kDa
• contiene: 15 mM sodium azide
• Reattività contro le specie: rat, goat, pig, human
• tecniche: microarray: suitable; western blot: 1:3,000 using fresh rat cerebellum extract
• Isotipo: IgG1
• N° accesso UniProt: P29475
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... NOS1(4842) ; rat ... Nos1(24598)

Descrizione

Descrizione generale

Monoclonal Anti-Nitric Oxide Synthase-Brain (bNOS) (mouse IgG1 isotype) is derived from the NOS-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Nitric oxide synthase (NOS) has been localized in many different cell types. Type I NOS is found in neurons. It is a 150-160 kDa protein, also called NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca²⁺- regulated NOS (cNOS). bNOS is present also in skeletal muscle, where it is complexed with dystrophin, and is absent in Duchenne′s muscular dystrophy, which perhaps accounts for symptoms of the disease.

Specificità

Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody is specific for nitric oxide synthase (NOS) derived from brain (bNOS, 150-160 kDa and several breakdown products of lower M.W.). The product does not bind to NOS derived from macrophages (mNOS) and endothelial cells (eNOS). This antibody is specific for bNOS in humans, goats, pigs and rats.

Immunogeno

recombinant neuronal NOS fragment (amino acids 1-181) from rat brain.

Applicazioni

Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse has been used in:

• western blot analysis
• immunocytochemistry
• double immunofluorescence
• immunofluorescence staining
• enzyme-linked immunosorbent assay (ELISA)
• dot blot immunoassay
• immunohistochemical staining

Azioni biochim/fisiol

Nitric oxide synthase is an enzyme that catalyzes the formation of nitric oxide. Nitric oxide is responsible for regulating several biochemical functions such as hemostasis, neurotransmission and vascular injury response . Mutations in nitric oxide synthase gene have been associated with diabetic nephropathy.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable