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MAK039

Sigma-Aldrich

Acetyl-Coenzyme A Assay Kit

sufficient for 100 fluorometric tests

Sinonimo/i:

Acetyl-CoA Assay Kit

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 fluorometric tests

Metodo di rivelazione

fluorometric

Malattie correlate

cancer; neurological disorders

Temperatura di conservazione

−20°C

Descrizione generale

Acetyl-CoA is an essential cofactor and carrier of acyl groups in enzymatic acetyl transfer reactions. It is formed either by the oxidative decarboxylation of pyruvate in mitochondria, by the oxidation of long-chain fatty acids, or by the oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound for the citric acid cycle (Kreb′s cycle). It is also a key precursor in lipid biosynthesis, and the source of all fatty acid carbons. Acetyl-CoA positively regulates the activity of pyruvate carboxylase. It is a precursor of the neurotransmitter acetylcholine. Histone acetylases (HAT) use Acetyl-CoA as the donor for the acetyl group used in the post-translational acetylation reactions of histone and non-histone proteins.

Acetyl-Coenzyme A acts as an indicator of fat, sugar, and protein levels and shows up on the nutritional status. Thus, starvation greatly reduces the level of acetyl-CoA, which stimulates the process of autophagy. In lowered glucose condition, acetyl-CoA participates in the production of ATP. Increased acetyl-CoA levels are observed in prostate cancer due to elevated fatty acid utilization and thus provides an additional energy source for the tumor cell growth. Nutrition deprivation, changes the levels of certain metabolites, including acetyl-CoA, which stimulates epigenetic modifications that would affect the central nervous system.

Applicazioni

Acetyl-Coenzyme A Assay Kit has been used to quantify the level of acetyl-CoA in astrocytes exposed to recombinant HIV protein Tat and Cocaine. It has also been used to study the mitochondrial metabolism by evaluating the levels of acetyl-CoA using hypoxia induced stress in primary human ventricular cardiomyocytes.

Compatibilità

Suitable for the measurement of Acetyl CoA in a variety of biological samples

Principio

Acetyl-CoA concentration is determined by a coupled enzyme assay, which results in a fluorometric (λex = 535/λem = 587 nm) product, proportional to the Acetyl-CoA present. Typical sensitivities of detection for this kit are 10-1000 pmole of Acetyl CoA. This kit is a highly sensitive assay for determining Acetyl-CoA level in a variety of biological samples.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

06693

Timestrip Plus™ -20 °C

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H315 - H319 - H335

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

188.6 °F - closed cup

Punto d’infiammabilità (°C)

87 °C - closed cup

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Lilong He et al.
Plant physiology, 180(1), 198-211 (2019-02-17)
Cadmium (Cd) is a major heavy metal pollutant, and Cd toxicity is a serious cause of abiotic stress in the environment. Plants protect themselves against Cd stress through a variety of pathways. In a recent study, we found that mitochondrial
Epigenetic mechanisms in neurological and neurodegenerative diseases.
Jorge L G, et al.
Frontiers in Cellular Neuroscience, 9, 58-58 (2015)
François Gagné et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 172-173, 36-44 (2015-05-11)
The purpose of this study was to determine the cumulative effects of exposure to either dissolved zinc or nanozinc oxide (nanoZnO) and air-time survival in freshwater mussels. Mussels were exposed to each forms of zinc for 96h then placed in
Maria I Dauden et al.
Science advances, 5(7), eaaw2326-eaaw2326 (2019-07-17)
The highly conserved Elongator complex modifies transfer RNAs (tRNAs) in their wobble base position, thereby regulating protein synthesis and ensuring proteome stability. The precise mechanisms of tRNA recognition and its modification reaction remain elusive. Here, we show cryo-electron microscopy structures
Nuclear-cytoplasmic localization of acetyl coenzyme a synthetase-1 in the rat brain
Ariyannur P S, et al.
The Journal of Comparative Neurology, 518(15), 2952-2977 (2010)
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