IPI-2I

93100622, Porcine intestine, Epithelial-like

• Codice UNSPSC: 41106514

Proprietà

• product name: IPI-2I, 93100622
• Origine biologica: Porcine intestine
• Modalità di accrescimento: Adherent
• Cariotipo: Heteroploid
• Morfologia: Epithelial-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Boar Miniature male ileum, SV40 transformed

Descrizione della linea cellulare

Epitheloid cell line derived from the ileum of an adult histocompatible miniature male boar. Transformed by SV40 plasmid pSV3-neo. Electron microscopy analysis shows evidence of microvilli and desmosomes connecting cells. The cells were found to co-express cytokeratin 18 specific of simple luminal epithelial cells and vimentin associated with a mesenchymal origin. Susceptible to Transmissible Gastro-enteritis (TG) virus.

Applicazioni

Studies of epithelial cell differentiation.

Terreno di coltura

DMEM + 4mM Glutamine + 0.024IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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