BLO-11

90112702

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse muscle
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 40
• Morfologia: Fibroblast-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse Moblo/y muscle hereditary emphysema

Descrizione della linea cellulare

Established from the abdominal wall muscle of a 30 day old male Blotchy mouse (Mo (blo/y)) with hereditary emphysema. The cells are deficient in lysyl oxidase activity and accumulate high levels of intracellular copper. NOR-10 (Sigma Catalogue number. 90112701) was isolated from the abdominal wall muscle of a normal litter mate of the mouse from which BLO-11 was derived, thus it can serve as a matched control for studies with BLO fibroblasts.

Applicazioni

Genetic studies

Terreno di coltura

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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