HT 1197

87032403, human bladder, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: HT 1197, 87032403
• Origine biologica: human bladder
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 46
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian bladder carcinoma

Descrizione della linea cellulare

Derived from a grade 4 transitional cell carcinoma of the bladder of a 44 year old Caucasian male who had not received chemo or radiation therapy. The cells grow in soft agar, produce fibrinolytic activity and are tumorigenic in mice and hamsters.

Applicazioni

HT 1197 has been used to study the transcription activity of human endogenous retroviruses (HERV).

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 16,18

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2x10,000 to 5x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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