C6992

Anti-CENP-A (C-terminal) antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-CenH3, Anti-Centromere Protein A
• Numero MDL: MFCD08705447
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen 17 kDa
• Reattività contro le specie: human
• Concentrazione: ~2 mg/mL
• tecniche: western blot: 1-2 μg/mL using HEK-293 cells expressing human CENP-A
• N° accesso UniProt: P49450
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CENPA(1058)

Descrizione

Descrizione generale

Centromere protein A is a protein encoded by the CENPA gene in humans. It is a histone H3 variant that has centromere-specific nucleosomal property and is located at neocentromere found on chromosome 10. CENP-A- binding clusters is associated with nucleosomal blocks within the neocentromeric chromatin.

Immunogeno

synthetic peptide corresponding to amino acids 124-140 located at the C-terminus of human CENP-A, conjugated to KLH. This sequence is highly conserved (88% identity) in bovine and dog CENP-A, and shows 60% identity with histone H3.

Applicazioni

Anti-CENP-A (C-terminal) antibody produced in rabbit is suitable for western blotting at a concentration of 1-2μg/mL using HEK-293 cells expressing human CENP-A.

Azioni biochim/fisiol

The location of the centromere on the chromosome is dependent on the histone H3 variant centromere protein A (CENP-A). The N-terminal tail of CENP-A is highly different from other H3 variants. Serine 7 of CENP-A plays an important role in mitosis-specific phosphorylation. It also plays a key role in kinetochore assembly. Alteration in CENP-A causes various human cancers. CENP-A may play an important role in epithelial ovarian cancer (EOC) and may serve as prognostic marker. It acts as a potential target for gene therapy in the treatment of EOC. The NH2 terminus of human CENP-A is essential for mitotic progression and necessary for the platform function of CENP-A centromeric chromatin in the assembly as well as maintenance of active kinetochores.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)