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MABF2761

Sigma-Aldrich

Anti-CXCL1/GROa Antibody, clone HL2401

Sinonimo/i:

C-X-C motif chemokine 1;GRO-alpha(1-73);Melanoma growth stimulatory activity;MGSA;Neutrophil-activating protein 3;NAP-3

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Prezzi e disponibilità al momento non sono disponibili

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

HL2401, monoclonal

PM

calculated mol wt 11.3 kDa
observed mol wt ~15 kDa

Purificato mediante

affinity chromatography

Reattività contro le specie

human

Confezionamento

antibody small pack of 100 μg

tecniche

ELISA: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
western blot: suitable

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SAB1403887SAB4301833ABN467
Anti-CXCL1 affinity isolated antibody

SAB4301833

Anti-CXCL1

Anti-CX3CL1 Antibody from rabbit, purified by affinity chromatography

ABN467

Anti-CX3CL1 Antibody

clone

HL2401, monoclonal

clone

2D7, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

mouse

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

mol wt

calculated mol wt 11.3 kDa, observed mol wt ~15 kDa

mol wt

antigen ~37.88 kDa

mol wt

-

mol wt

-

Gene Information

human ... CXCL1(2919)

Gene Information

human ... CXCL1(2919)

Gene Information

human ... CXCL1(2919)

Gene Information

mouse ... Cx3Cl1(20312)

Descrizione generale

Growth-regulated alpha protein (UniProt: P09341; also known as C-X-C motif chemokine 1, CXCL1, GRO-alpha(1-73), Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3) is encoded by the CXCL1 (also known as GRO, GRO1, GROA, MGSA, SCYB1) gene (Gene ID: 2919) in human. CXCL1 is a multi-functional chemokine that displays chemotactic activity for neutrophils. It is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to produce the mature protein that can be further cleaved into three chains (aa 4-73; 5-73; and 6-73) via proteolytic cleavage after secretion from peripheral blood monocytes. CXCL1 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. Its levels are reported to be low under basal conditions but increase significantly following active infection. It plays a role in recruiting and activating neutrophils for microbial killing at the tissue site and can also activate the release of proteases and reactive oxygen species. CXCL1 signals through binding to CXCR2, a G-protein coupled receptor. Aberrant expression of CXCL1 has been reported in several cancers and has been associated with oncogenesis, metastasis, angiogenesis and chemoresistance. Clone HL2401 is shown to specifically binds to CXCL1, although it may show slight binding to CXCL2. It is shown to reduce both cellular proliferation and invasion of cancer cells overexpressing CXCL1. It also induces apoptosis and impairs tumor vasculature. (Ref.: Miyake, M., et al. (2019). Theranostics.;9(3):853-867; Yang C., et al. (2019). Int. J. Oncol. 55(3); 684-696Wuyts, A., et al. (1999). Eur. J. Biochem. 260(2); 421-429).

Specificità

Clone HL2401 is a mouse monoclonal antibody that detects CXCL1.

Immunogeno

Native human CXCL1 protein.

Applicazioni

Quality Control Testing

Evaluated by Western Blotting with recombinant Human CXCL1 protein.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant Human CXCL1 protein.

Tested Applications

ELISA Analysis: A representative lot detected CXCL1 in ELISA application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

Inhibition Assay: A representative lot inhibited proliferation and invasion of bladder and prostate cells and disrupted endothelial sprouting in vitro. (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CXCL1 in Immunoprecipitation application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

Western Blotting Analysis: A representative lot detected CXCL1 in Western Blotting application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Stato fisico

Purified mouse monoclonal antibody IgG1 in PBS with 0.02% sodium azide.

Ricostituzione

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommended storage: +2°C to +8°C.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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