23-026-M

SCF-TrCP1 Protein Complex, Active, 10 µg

Active complex of four (4) recombinant human enzymes: full length Skp1, full length Cul1, full length Rbx1, & βTrCP1 (amino acids 53-end). For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

• Sinonimo/i: SCF-β-TrCP, SCF ^FWD1, SCF-bTrCP1
• Codice UNSPSC: 12352200
• eCl@ss: 32160405
• NACRES: NA.41

Proprietà

• Origine biologica: human
• Ricombinante: expressed in E. coli
• Produttore/marchio commerciale: Upstate^®
• tecniche: activity assay: suitable
• N° accesso NCBI: NM_003592; NM_014248; NM_033637; NM_170679

Descrizione

Descrizione generale

N-terminal GST-tagged, recombinant human Smurf1 full length, expressed in E coli.

The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCF^βTrCP1 mediates the ubiquitination and subsequent proteasomal degradation of a variety of proteins involved in various cellular processes, these include IκBα, an inhibitor of the transcription factor NFκB and β-catenin which is involved in the Wnt signalling pathway. A number of cancers, including those in breast, colon and pancreatic tissue have been associated with the deregulation of SCF^βTrCP1

Applicazioni

Ubiquitination Cascade Comoponent: E3

Stato fisico

Purified using glutathione sepharose.

Altre note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H317
• Consigli di prudenza: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classi di pericolo: Skin Sens. 1
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable