1.19613

Superspher^® 100 RP-18 (4 µm) HPLC Columns

HPLC sorbent

• Sinonimo/i: TM=["Superspher"] C18 sorbent for HPLC
• Codice UNSPSC: 41115709
• NACRES: SB.52

Proprietà

• product name: Superspher^® 100 RP-18,
• Materiali: stainless steel column
• agenzia: suitable for USP L1
• Nome Commerciale: Superspher^®
• Caratteristiche: endcapped: no
• Parametri: 60 °C max. temp.
• tecniche: HPLC: suitable; mass spectrometry (MS): suitable (Low - Medium)
• Matrice: fully porous particle
• Gruppo funzionale matrice: C18 bonding phase
• Dimensione particelle: 4 μm
• Dimensione pori: 100 Å pore size
• operating pH range: 2-7.5
• Densità bulk: 200‑400 kg/m³
• Tecnica di separazione: reversed phase
• Temperatura di conservazione: no temp limit

Descrizione

Descrizione generale

Superspher^® RP-18 is a reliable and versatile traditionally produced spherical silica gel carriers with C-18 reversed phase properties. They are well suited for the separation of neutral, acidic and weak basic compounds. This high performance silica carrier with a particle size of 4µm yields an excellent pressure/separation performance ratio.

Applicazioni

• Determination of retinol and retinyl esters in human plasma: This study utilized high-performance liquid chromatography with automated column switching and ultraviolet detection, highlighting the utility of the Superspher 100 RP-18 HPLC column in sensitive biochemical analyses (Hartmann et al., 2001).


• Enantioselective assay for ketoprofen glucuronidation: This study underscores the column′s capability in pharmacokinetic research, using HPLC to analyze chiral anti-inflammatory drugs and their metabolites in liver microsomes (Chakir et al., 1994).


• Rapid liquid chromatographic assay of glutathione in cultured cells: Employing the Superspher 100 RP-18 column, this research focused on rapid and effective glutathione quantification, demonstrating the column′s versatility in biomedicine (Leroy et al., 1993).


• Assay of chloramphenicol acetyl transferase by high-performance liquid chromatography: This application further exemplifies the adaptability of Superspher^® 100 RP-18 in biotechnology, offering precise measurement techniques for enzymatic activity studies (Burzio et al., 1988).

Risultati analitici

Application test: passes test
Pressure: 50 - 70 bar
Theoretical Plates (N/m) (Anthracene): ≥ 105000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.0 - 5.5
separation factor (Acetophenone/Phenol, selectivity test): 1.90 - 2.15
separation factor (Acetophenone/Cinnamic acid, selectivity test): 1.20 - 1.37
separation factor (1-Naphtol/Phenol, selectivity test): 3.80 - 4.30
capacity factor (Toluene): 17.0 - 22.0

Note legali

Superspher is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable