05-1403

Anti-Giα2 Antibody, clone L5

clone L5, from mouse

• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: L5, monoclonal
• Reattività contro le specie: guinea pig (43%), rat (69%), bovine (100%), human (62%), mouse (69%)
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2b
• N° accesso NCBI: NM_002070.2
• N° accesso UniProt: P04899
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GNAI2(2771)

Descrizione

Specificità

Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.

This antibody recognizes Giα-2.

Immunogeno

Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).

Applicazioni

Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer

Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.

Qualità

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.

Descrizione del bersaglio

Labels a single protein band of 39-42 kDa in bovine and rat membranes.

Linkage

Replaces: MAB3077

Stato fisico

Format: Purified

Purified

Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.

Stoccaggio e stabilità

Maintain at 2-8°C for 1 year from date of receipt.

Risultati analitici

Control
Huvec cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable