Microcon^® Centrifugal Filters Applications Guidelines & Specifications

Microcon^® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes. With the low- binding Ultracel^® membrane, Microcon^® filters offer:

• Typical recoveries of >95%, even for dilute solutions
• Reverse spin to maximize recovery, even in the smallest samples
• Convenient storage of filtrate or concentrated sample in standard microfuge tube.

The Microcon^® DNA Fast Flow Filter is optimized for the concentration and recovery of genomic DNA. The low non-specific binding characteristics of the membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss. With its inverted recovery spin, the device can concentrate samples up to 100X (for greatest consistency and reproducibility, we recommend concentration factors of < 20X).

Applications Guidelines

Use the following table to choose the correct Microcon^® device for your application.

	Microcon® Device
Application	10K	30K	DNA Fast Flow
Peptide and growth factor concentration	●
Protein concentration and desalting of columns eluates	●	●
Protein concentration before electrophoresis or other assays	●	●
Protein removal prior to HPLC	●	●
Purification of macromolecular components found in tissue culture extracts and cell lysates	●	●
Concentration of biological samples (antigens, antibodies, enzymes)		●
Concentration and desalting of nucleic acids (single-or double-stranded)	●	●	●
Removal of labeled nucleotides	●	●	●
Removal of labeled amino acids	●	●	●
Removal of primers from amplified DNA		●	●
Removal of linkers prior to cloning		●	●

Specifications

Maximum initial sample volume	0.5 mL (500 μL)
Typical final concentrate volume	5–50 μL
Maximum relative centrifugal force
Microcon® 10K devices	14,000 × g
Microcon® 30K devices	14,000 × g
Microcon® DNA Fast Flow devices	500 × g
Active membrane area	0.32 cm2
Hold-up volume	≤ 10 μL

Ordering Information