Ascentis^® Express F5 (Pentafluorophenyl) U/HPLC Columns

Enhanced selectivity for stereoisomers and closely related compounds

Our Ascentis^® Express F5 column brings together the highly efficient, robust Fused-Core^® particle technology with F5 chemistry for the successful separation of polar and non-polar organic compounds.

The pentafluorophenylpropyl (PFP) stationary phase of the Ascentis^® Express F5 column, based on Fused-Core^® particle technology, provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis^® Express F5 columns can be used for basic, acidic, or neutral compounds with alternate selectivity from C18 phases.

Ascentis® Express F5 Specifications:
Silica:	Type B (High purity silica)
Particle Platform:	Superficially porous particles (SPP)
Phase Chemistry:	Pentafluorophenylpropyl
Particle Size:	2.0 µm / 2.7 µm / 5.0 µm
Pore Size:	90 Å
Carbon Load:	5.3% / 5.5% / 3.9%
Surface Area:	120 m²/g / 135 m²/g / 90 m²/g
pH Range:	2 - 9
Max Temperature at Low pH:	60 °C
Max Temperature at High pH:	40 °C
Endcapped:	Yes

Learn More About Fluorinated Stationary Phases:

• Alternative Retention and Selectivity Using Fluorinated Stationary Phases

Comparision of C18 and F5 for the Separation of Closely Related Steroids

Columns	Ascentis® Express F5 (53590-U) and Ascentis® Express C18 (53827-U), 10 cm x 4.6 mm I.D, 2.7 μm particle size
Mobile phase A:	Water
Mobile phase B:	methanol
Mobile phase ratio:	A:B 50:50, v/v
Flow rate:	0.8 mL/min
Temp.	35 ºC
Pressure:	~2400 psi
Det.:	UV at 240 nm
Injection:	5 μL
Sample:	10 μg/mL each in 90:10 water:methanol

Featured Applications:

• Fast and Accurate LC-MS Analysis of Vitamin D Metabolites Using Ascentis^® Express F5 HPLC Columns
• Anesthetics on Ascentis^® Express F5
• Selegiline and Amphetamines on Ascentis^® Express F5 (20/80 mobile phase mix)
• 25-dihydroxyvitamin D2, 25-hydroxyvitamin D3 and 3-epi-25-hydroxyvitamin D3 on Ascentis Express F5
• 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 on Ascentis^® Express F5
• 25-hydroxyvitamin D3 and 3-epi-25-hydroxyvitamin D3 on Ascentis^® Express F5
• Analysis of Steroids using Ascentis^® Express F5
• Analysis of Taxols Using Ascentis^® Express F5

Ascentis^® Express F5 is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core^® particle design. The Fused-Core^® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths. 3 different particle sizes are available for your specific needs:

2.0 µm particles

Best Fused-Core UHPLC Column

An optimized solution for high-throughput small molecule analysis

2.7 µm particles

Fast HPLC on ANY System

A practical solution that delivers UHPLC performance from any HPLC

5.0 µm particles

The Lab Work-Horse Column

True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns