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HPLC Analysis of Sugars on Ascentis® Si

HPLC Analysis of Sugars on Ascentis® Si application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

581512-U

Colonne HPLC Ascentis® Si

5 μm particle size, L × I.D. 15 cm × 4.6 mm

Étalon

Réf. du produit
Description
Tarif

84097

Saccharose

BioUltra, for molecular biology, ≥99.5% (HPLC)

47287-U

Lactose Monohydrate

analytical standard

G0350500

D-(+)-Glucose

European Pharmacopoeia (EP) Reference Standard

X0200000

Xylose

European Pharmacopoeia (EP) Reference Standard

CONDITIONS

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

Description

Remarque sur l'analyse

This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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