HPLC Analysis of Salicylic Acid and Acetylsalicylic Acid (Aspirin) on Ascentis^® Express OH5

column

Ascentis Express OH5, 10 cm x 3.0 mm I.D., 2.7 μm particles (53769-U)

mobile phase

2 mM ammonium acetate in 10:90 water:acetonitrile, pH to 7.0 with acetic acid, glacial

flow rate

0.5 mL/min

pressure

850 psi (59 bar)

column temp.

35 °C

detector

UV, 235 nm

injection

2 μL

sample

100 μg/mL salicylic acid, 200 μg/mL acetylsalicylic acid in methanol:acetonitrile (3:7)