HPLC Analysis of Mephobarbital Enantiomers on Astec® Cellulose DMP
CONDITIONS
column
Astec Cellulose DMP; 25 cm x 4.6 mm I.D., 5 μm particles (51099AST)
mobile phase
[A] heptane; [B] isopropanol; (80:20, A:B)
flow rate
1 mL/min
column temp.
30 °C
detector
UV, 254 nm
injection
15 μL
sample
mephobarbital, 1 g/L in 50:50, heptane:isopropanol
Description
Remarque sur l'analyse
Mephobarbital enantiomers are resolved by normal phase chromatography.
Autres remarques
Mephobarbital (aka methyphenobarbital) is used as a sedative, anxiolytic and anticonvulsant. It is the N-methylated analogue of phenobarbital, and has similiar indications, therapeutic value, and tolerability.
Informations légales
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany