HPLC Analysis of Free Fatty Acids on SUPELCOSIL^™ LC-8

column

SUPELCOSIL LC-8, 15 cm × 4.6 mm I.D., 3 μm particles (58983)

mobile phase

acetonitrile:tetrahydrofuran: 0.1% phosphoric acid (50.4:21.6:28)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 215 nm

injection

10 μL