HPLC Analysis of Crufomate (Ruelene) Enantiomers on Astec^® CYCLOBOND I 2000

column

CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)

mobile phase

[A] acetonitrile; [B] acetic acid; [C] triethylamine; (100:0.2:0.1, A:B:C)

flow rate

1 mL/min

column temp.

ambient

detector

UV, 254 nm

injection

5 μL

sample

1 mg/mL in methanol