Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis.

Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis.

The Analyst (2017-06-16)

K Lipponen, S Tähkä, T Sikanen, V Jokinen, A Tatikonda, S Franssila, R Kostiainen, T Kotiaho

RÉSUMÉ

A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol-ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous-organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10

MATÉRIAUX

Référence du produit

Marque

Description du produit

381489

Sigma-Aldrich

Trimethylolpropane tris(3-mercaptopropionate), ≥95.0%

469319

Sigma-Aldrich

Poly(dimethylsiloxane), viscosity 1.0 cSt (25 °C)