Accéder au contenu
MilliporeSigma

Lactulose and Lactobacillus plantarum, a potential complementary synbiotic to control postweaning colibacillosis in piglets.

Applied and environmental microbiology (2014-06-08)
A A Guerra-Ordaz, G González-Ortiz, R M La Ragione, M J Woodward, J W Collins, J F Pérez, S M Martín-Orúe
RÉSUMÉ

The potential of a prebiotic oligosaccharide lactulose, a probiotic strain of Lactobacillus plantarum, or their synbiotic combination to control postweaning colibacillosis in pigs was evaluated using an enterotoxigenic Escherichia coli (ETEC) K88 oral challenge. Seventy-two weanlings were fed four diets: a control diet (CTR), that diet supplemented with L. plantarum (2 × 10(10) CFU · day(-1)) (LPN), that diet supplemented with 10 g · kg(-1) lactulose (LAC), or a combination of the two treatments (SYN). After 7 days, the pigs were orally challenged. Six pigs per treatment were euthanized on days 6 and 10 postchallenge (PC). Inclusion of lactulose improved the average daily gain (ADG) (P < 0.05) and increased lactobacilli (P < 0.05) and the percentage of butyric acid (P < 0.02) in the colon. An increase in the ileum villous height (P < 0.05) and a reduction of the pig major acute-phase protein (Pig-MAP) in serum (P < 0.01) were observed also. The inclusion of the probiotic increased numbers of L. plantarum bacteria in the ileum and colon (P < 0.05) and in the total lactobacilli in the colon and showed a trend to reduce diarrhea (P = 0.09). The concentrations of ammonia in ileal and colonic digesta were decreased (P < 0.05), and the villous height (P < 0.01) and number of ileal goblet cells (P < 0.05) increased, at day 10 PC. A decrease in plasmatic tumor necrosis factor alpha (TNF-α) (P < 0.01) was also seen. The positive effects of the two additives were combined in the SYN treatment, resulting in a complementary synbiotic with potential to be used to control postweaning colibacillosis.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Lactulose, ≥98.0% (HPLC)
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Sigma-Aldrich
Lactulose, ≥95% (HPLC)
Le tarif et la disponibilité ne sont pas disponibles actuellement.
USP
Lactulose, United States Pharmacopeia (USP) Reference Standard
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Supelco
Lactulose, Pharmaceutical Secondary Standard; Certified Reference Material
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Lactulose, European Pharmacopoeia (EP) Reference Standard
Le tarif et la disponibilité ne sont pas disponibles actuellement.