Avanti lipid tools: connecting lipids, technology, and cell biology.

Avanti lipid tools: connecting lipids, technology, and cell biology.

Biochimica et biophysica acta (2014-06-24)

Kacee H Sims, Ewan M Tytler, John Tipton, Kasey L Hill, Stephen W Burgess, Walter A Shaw

RÉSUMÉ

Lipid research is challenging owing to the complexity and diversity of the lipidome. Here we review a set of experimental tools developed for the seasoned lipid researcher, as well as, those who are new to the field of lipid research. Novel tools for probing protein-lipid interactions, applications for lipid binding antibodies, enhanced systems for the cellular delivery of lipids, improved visualization of lipid membranes using gold-labeled lipids, and advances in mass spectrometric analysis techniques will be discussed. Because lipid mediators are known to participate in a host of signal transduction and trafficking pathways within the cell, a comprehensive lipid toolbox that aids the science of lipidomics research is essential to better understand the molecular mechanisms of interactions between cellular components. This article is part of a Special Issue entitled Tools to study lipid functions.

MATÉRIAUX

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