Accéder au contenu
Merck
  • Ligands playing musical chairs with G-quadruplex DNA: a rapid and simple displacement assay for identifying selective G-quadruplex binders.

Ligands playing musical chairs with G-quadruplex DNA: a rapid and simple displacement assay for identifying selective G-quadruplex binders.

Biochimie (2008-03-18)
D Monchaud, C Allain, H Bertrand, N Smargiasso, F Rosu, V Gabelica, A De Cian, J-L Mergny, M-P Teulade-Fichou
RÉSUMÉ

We report here the details of G4-FID (G-quadruplex fluorescent intercalator displacement), a simple method aiming at evaluating quadruplex-DNA binding affinity and quadruplex- over duplex-DNA selectivity of putative ligands. This assay is based on the loss of fluorescence upon displacement of thiazole orange from quadruplex- and duplex-DNA matrices. The original protocol was tested using various quadruplex- and duplex-DNA targets, and with a wide panel of G-quadruplex ligands belonging to different families (i.e. from quinacridines to metallo-organic ligands) likely to display various binding modes. The reliability of the assay is further supported by comparisons with FRET-melting and ESI-MS assays.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
PhenDC3, ≥97% (HPLC)