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Merck

Use of exogenous volatile organic compounds to detect Salmonella in milk.

Analytica chimica acta (2018-06-10)
Najat H O Bahroun, John D Perry, Stephen P Stanforth, John R Dean
RÉSUMÉ

Rapid, sensitive, and selective detection and identification of pathogenic bacteria is required in terms of food security. In this study, exogenous VOCs liberated by Salmonella strains have been identified and quantified via head space-solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GC-MS) in milk samples. The specific enzymes targeted for detection and/or differentiation of Salmonella were C8 esterase, α-galactosidase and pyrrolidonyl peptidase using the following enzyme substrates: 2-chlorophenyl octanoate, phenyl α-d-galactopyranoside and L-pyrrollidonyl fluoroanilide, respectively. Detection of the exogenous VOCs, 2-chlorophenol, phenol and 3-fluoraniline was possible with typical limits of detection of 0.014, 0.045 and 0.005 μg/mL, respectively and correlation coefficients >0.99. The developed methodology was able to detect and identify Salmonella species within a 5 h incubation at 37 °C by the detection of the liberated VOCs. It was found that the milk samples tested were Salmonella free.

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Description du produit

Supelco
Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, StableFlex
Supelco
Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler
Supelco
Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder
Supelco
Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm
Supelco
Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler
Supelco
Fibres SPME Polydimethylsiloxane (PDMS), df 100 μm(PDMS, for use with autosampler, needle size 23 ga
Supelco
Fibres SPME Polydimethylsiloxane (PDMS), df 100 μm(PDMS, needle size 24 ga, for use with manual holder
Sigma-Aldrich
L-Pyroglutamic acid, ≥99.0% (T)
Supelco
Fibres SPME Polydimethylsiloxane (PDMS), df 100 μm(PDMS, for use with autosampler, needle size 24 ga
Supelco
Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), df 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga
Sigma-Aldrich
Octanoyl chloride, 99%
Supelco
Unité de fibres SPME, Polydiméthylsiloxane/divinylbenzène (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with autosampler
Supelco
Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler
Supelco
Unité de fibres SPME, Polydiméthylsiloxane/divinylbenzène (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, for use with manual holder
Supelco
Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler
Supelco
Fibres SPME polyacrylate (PA), df 85 μm(PA, for use with autosampler, needle size 23 ga
Supelco
Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler
Supelco
Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber
Supelco
Unité de fibres SPME, Polydiméthylsiloxane/divinylbenzène (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 23 ga, PDMS/DVB StableFlex, for use with autosampler
Supelco
Fibres SPME polyacrylate (PA), df 85 μm(PA, for use with autosampler, needle size 24 ga
Supelco
Unité de fibres SPME, Polydiméthylsiloxane/divinylbenzène (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, for use with autosampler
Supelco
Unité de fibres SPME, Polydiméthylsiloxane/divinylbenzène (PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with manual holder
Supelco
Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler
Supelco
Fibres SPME Polydimethylsiloxane (PDMS), df 100 μm(PDMS, for use with manual holder, needle size 23 ga
Supelco
Unité de fibres SPME, Polydiméthylsiloxane/divinylbenzène (PDMS/DVB), df 65 μm(PDMS/DVB, for use with autosampler, needle size 23 ga
Supelco
SPME StableFlex fiber assortment kit, for use with manual holder
Supelco
Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder
Supelco
SPME fiber assembly, Carbowax-Polyethylene Glycol (PEG) Coating, needle size 23 ga, df 60 μm(PEG, for use with autosampler
Supelco
SPME StableFlex fiber assortment kit, needle size 23 ga, for use with autosampler
Supelco
Unité de fibres SPME, Polydiméthylsiloxane/divinylbenzène (PDMS/DVB), fused silica fiber, df 65 μm(PDMS/DVB, for use with manual holder, needle size 23 ga