ZRB2221

Anti-NKX2-1 Antibody, clone 1H5-H2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Anti-HIF-2α/EPAS1 Antibody, clone 2L21 ZooMAb^® Rabbit Monoclonal, Homeobox protein NK-2 homolog A, Homeobox protein Nkx-2.1, T/EBP, TTF-1, Thyroid nuclear factor 1, Thyroid transcription factor 1, Thyroid-specific enhancer-binding protein
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1H5-H2, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 38.60 kDa; observed mol wt ~40 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse, porcine, bovine, rat, canine, monkey
• Conditionnement: antibody small pack of 25
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: N-terminal half
• Numéro d'accès Protein ID: NP_003308
• Numéro d'accès UniProt: P43699
• Autre catégorie plus écologique: Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... NKX2-1(7080)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1H5-H2 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Homeobox protein Nkx-2.1 (NKX2-1). It targets an epitope within 16 amino acids from the N-terminal half.

Immunogène

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human Homeobox protein Nkx-2.1 (NKX2-1).

Application

Quality Control Testing

Evaluated by Western Blotting in TT cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NKX2-1 in TT cell lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected NKX2-1 in Human thyroid tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound NKX2-1 peptide with a KD of 2.7 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NKX2-1 in TT cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Homeobox protein Nkx-2.1 (UniProt: P43699; also known as Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, TTF-1, Thyroid-specific enhancer-binding protein, T/EBP) is encoded by the NKX2-1 (also known as NKX2A, TITF1, TTF1) gene (Gene ID: 7080) in human. NKX2-1 is a member of the NK-2 homeobox family of transcription factors that is selectively expressed in lung and thyroid tissue. It is considered as one of the earliest markets of lung development. It is shown to be of fundamental importance in driving lung maturation and morphogenesis. Mice deficient in NKX2-1 fail to undergo tracheoesophageal septation. It is reported to bind and activate the promoter of thyroid specific genes, such as thyroglobulin, thyroperoxidase, and thyrotropin receptor and is crucial in the maintenance of the thyroid differentiation phenotype. NKX2-1 null mice display a perinatal lethal phenotype characterized by severe defects in both lung and forebrain development as

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable