ZRB1659

Anti-phospho-HSP27 Ser82 Antibody, clone 1O17 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Heat shock protein beta-1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1O17, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 22.78 kDa; observed mol wt ~30 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): bovine, horse, mouse, feline, monkey, canine, rat
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Isotype: IgG
• Séquence de l'épitope: N-terminal half
• Numéro d'accès Protein ID: NP_001531
• Numéro d'accès UniProt: P04792
• Autre catégorie plus écologique: Aligned,
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: phosphorylation (pSer82)
• Informations sur le gène: human ... HSPB1(3315)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1O17 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects HSP27 phosphorylated on serine 82.

Immunogène

KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphoserine 82 from the N-terminal half of human Heat shock protein 27 (HSP27).

Application

Quality Control Testing

Evaluated by Western Blotting in lysates from HeLa cells overnight serum starved and treated with Anisomycin.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected HSP27 phosphorylated on serine 82 in lysates of HeLa cells overnight serum starved and treated with Anisomycin (25 μg/mL; 30 min.).

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected phospho-HSP27 Ser82 in lysate from UV-treated HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-HSP27 Ser82 in human small intestine and human colon cancer tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound phospho-HSP27-ser82 peptide with a KD of 1. x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-HSP27 Ser82 in HeLa cells treated with Anisomycin.

Peptide Inhibition Assay: Target band detection in lysate from HeLa cells treated with Anisomycin was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Description de la cible

Heat shock protein beta-1 (UniProt: P04792; also known as HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27) is encoded by the HSPB1 (also known as HSP27, HSP28) gene (Gene ID: 3315) in human. Heat shock proteins (HSPs) are produced during nonlethal stress conditions and protect the organism from severe and sometimes lethal stress. HSPs can also be induced by non-heat related stress factors. HSP27 is a multidimensional protein that acts as a protein chaperone and an antioxidant and plays a role in the inhibition of apoptosis and actin cytoskeletal remodeling. It is detected in all tissues; however, its highest levels are found in the heart and in tissues composed of striated and smooth muscle. It is shown to be mostly cytoplasmic in interphase cells and is shown to colocalize with mitotic spindles in mitotic cells. It translocates to the nucleus during heat shock and resides in sub-nuclear structures known as nuclear splicing speckles and functions to stabilize DNA and/or the nuclear membrane. HSP-27 contains a conserved -crystalline homology domain at its C-terminal. It is phosphorylated upon exposure to protein kinase C activators and heat shock. HSP27 is present at basal levels in cells and tissues and is organized as large oligomers. After heat shock, exposure to chemical stress, or addition of steroid hormones, its synthesis increases several fold. It is phosphorylated by MAPKAP kinase 2/3 via the activation of the p38 MAPK pathway at multiple serine residues. Following phosphorylation, HSP27 reorganizes itself into smaller oligomers that interact with other proteins. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Alderson TR., et al. (2019). Nat. Commun. 10; Article 1068; Vidyasagar, A., et al. (2012). Fibrogenesis Tissue Repair. 5(1); 7; Landry, J., et al. (1992). J. Biol. Chem. 267(2); 794-803).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable