SRP6382

CD304/NRP1/Neurophilin-1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonyme(s) : NRP, VEGF165R
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in HEK 293 cells
• Étiquette/Marqueur: 6-His tagged (C-terminus)
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: calculated mol wt 70.7 kDa; observed mol wt 85-90 kDa (DTT-reduced. Protein migrates due to glycosylation. Phe 22 is the predicted N-terminus.)
• Conditionnement: pkg of 10 μg
• Numéro d'accès UniProt: O14786
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... NRP1(8829)

Description

Description générale

Neuropilin-1 (NRP1) is also known as vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The NRP1 gene is located on human chromosome 10p11.22.

Actions biochimiques/physiologiques

Neuropilin-1 (NRP1) is membrane spanning protein that serves as coreceptor for a number of growth factors such as vascular endothelial growth factor, transforming growth factor β1, hepatocyte growth factor and platelet-derived growth factor. It is known to mediate immune responses, wound healing and angiogenesis, epithelial to mesenchymal transition in cancer. Mutation in the NRP1 gene results in tetralogy of Fallot (congenital heart disease).
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, tThe PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells

Forme physique

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable