SRP0723

YSK4 (MAP3K19), Active

PRECISIO^® Kinase, recombinant

• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Pureté: 70-95% (SDS-PAGE)
• Forme: frozen liquid
• Activité spécifique: 30-45 nmol/min·mg (radiometric)
• Poids mol.: ~83 kDa
• Concentration: 0.05 μg/μL
• Numéro d'accès: NM_025052
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... YSK4(80122)

Description

Application

Useful for kinase assay and Western blot.

Actions biochimiques/physiologiques

Recombinant human YSK4 (MAP3K19) (941-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. YSK4 (MAP3K19, RCK) belongs to STE Ser/Thr protein kinase family and STE20 subfamily. The YSK4 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat ⁽¹⁾. The important paralog is MAP3K1 gene. The RCK ligand-binding domains form an octameric gating ring ⁽²⁾ in the large-conductance Ca²⁺-activated K⁺ channels ⁽³⁾. The YSK4 kinase function has not been made clearly yet.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Stockage et stabilité

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Informations légales

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable