SRP0192

PARP1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Synonyme(s) : ADPRT, NAD(+) ADP-ribosyltransferase 1, Poly (ADP-ribose) Polymerase 1
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥80% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 140 kDa
• Conditionnement: pkg of 10 and 20 μg
• Fabricant/nom de marque: Sigma-Aldrich
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: >0.02 mg/mL
• Technique(s): inhibition assay: suitable
• Numéro d'accès NCBI: NM_001618
• Numéro d'accès UniProt: P09874
• Application(s): life science and biopharma
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PARP1(142)

Description

Description générale

Research area: Cell Signaling

Human PARP1 (GenBank Accession No. NM_001618), full length with N-terminal GST tag, MW = 140 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Poly [ADP-ribose] polymerase 1 (PARP1) belongs to the DNA-dependent nuclear enzyme superfamily. Its structure includes an N-terminal with three zinc finger DNA-binding domains, an auto-modification domain with BRCT motif and a WGR domain having conserved tryptophan, glycine, and arginine residues, and a C-terminal catalytic domain with PARP signature sequence.

Application

PARP1 Active human has been used in GST-PARP1 pulldown, to study the alterations in protein-protein interactions of sex determining region Y-box 2 (SOX2) upon O-GlcNAcylation. It has also been used in in vitro PARylation assay to investigate the effect of NR1D1 on DNA repair after damage induced by doxorubicin in breast cancer cells. It is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

PARPs comprise a set of enzymes that govern cellular processes such as DNA damage response, cell metabolism, chromatin remodeling, and transcriptional regulation. PARP1 detects and mends DNA breaks via base excision repair when DNA damage occurs. In the absence of PARP1 activity, damaged DNA accumulates, leading to impaired DNA replication.

Définition de l'unité

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable