MAK193
Citrate Synthase Activity Assay Kit
Sufficient for 100 Colorimetric tests
Synonyme(s) :
Citrate Synthase Test Kit
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About This Item
Produits recommandés
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
endocrinological disorders, diabetes; cancer
Température de stockage
−20°C
Informations sur le gène
human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)
Description générale
Citrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern.
Application
Citrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples.
Adéquation
Suitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
Principe
The Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2.
Composants de kit seuls
Réf. du produit
Description
- CS Assay Buffer
- CS Substrate Mix
- CS Developer
- GSH Standard, Reduced
- CS Positive Control
Remplacé(e)(s) par
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
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Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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