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Key Documents

HCLHPV

Sigma-Aldrich

HPV/p16 Analyte Control

Cell line controls for immunohistochemistry and in situ hybridization

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.47

Source biologique

human cell culture

Conditionnement

pkg of 1 ea cell microarray block (HCL006)
pkg of 2 slides (HCL004)
pkg of 5 slides (HCL005)

Fabricant/nom de marque

Histocyte

Température de stockage

2-8°C

Description générale

The HPV/p16 Analyte Control has three cell line cores which demonstrate high, medium, and negative expression of high risk human papillomavirus (HPV) types 16 and 18. The same cell lines also demonstrate high homogenous, high heterogenous, and negative expression of p16.
HistoCyte Laboratories control slides and blocks are made from cultured human cell lines to provide the required range of expression of a specific biomarker. The cell lines are processed using proprietary methods that allow for the creation of a high density embedded cell block with cores that retain their cellular morphology. Available as pre-cut unstained slides or as cell microarray blocks for sectioning.

Application

Ideal for use as a same slide control for HPV in situ hybridization (ISH) and p16 immunohistochemistry (IHC).
The HPV/p16 Analyte Control histology slides have three cell line cores providing a range of expression for HPV type 16 and 18: high, medium, and negative. The same cell lines also demonstrate high homogenous, high heterogenous and negative expression of p16. Analyte controls are placed at one end of slide, allowing sample application on same slide for valid comparison.
The HPV/p16 Analyte Control cell microarray block contains three cell line cores that are 2 mm in diameter and between 3.0-3.5 mm in length. The cell microarray block may yield over 300 sections depending on thickness.

Notes préparatoires

Cell microarray blocks are fixed in 10% neutral buffered formalin and embedded in paraffin wax. Pre-cut unstained slides are prepared from 4 μm thick sections, mounted on positively charged slides, and dried at 37 °C overnight.

Autres remarques

For certificates of analysis, contact info@histocyte.com.
HPV/p16 Analyte Control brochure is available from HistoCyte Laboratories.

Certificats d'analyse (COA)

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