GE17-5130-01

GSTrap^™ Fast Flow

Cytiva 17-5130-01, pack of 5 × 1 mL

• Synonyme(s) : GSTrap column
• Code UNSPSC : 23151817
• Nomenclature NACRES : NA.25

Propriétés

• ligand: glutathione and 10-carbon linker arm
• Conditionnement: pack of 5 × 1 mL
• Fabricant/nom de marque: Cytiva 17-5130-01
• Paramètres: flow rate; 42 psi
• Taille du lit: 7 mm × 25 mm
• Volume du lit: 1 mL
• D.I. de la colonne: 7 mm
• Matrice: highly cross-linked 4% agarose
• Diamètre moyen: 90 μm
• cleaning: 3-12
• working range: 3-12
• Capacité: >10 mg/mL binding capacity

Description

Description générale

GSTrap^™ FF columns are prepacked Glutathione Sepharose^™ Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC^™ System.

* See the section for licensing information in our terms and conditions.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Caractéristiques et avantages

• Fast and simple one-step purification of GST-tagged proteins.
• Mild elution conditions preserving protein antigenicity and function.
• Easy scale-up by connecting the columns in series.
• Sepharose^™ 4 Fast Flow provides good flow properties.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

FPLC is a trademark of Cytiva

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids