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Key Documents

GE17-5087-02

Sigma-Aldrich

HiPrep™ 26/10 Desalting

Cytiva 17-5087-02, column L × I.D. 10 cm × 26 mm, 17-132 μm particle size (dry)

Synonyme(s) :

Desalting Column, HiPrep Desalting Column

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About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

Matériaux

polypropylene (PP)

Poids mol.

1000-5000 Da (globular proteins)

Conditionnement

pack of 4

Fabricant/nom de marque

Cytiva 17-5087-02

Conditions de stockage

dry media

Paramètres

<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)

Volume du lit

53 mL

Colonne, L × D.I.

10 cm × 26 mm

Matrice

cross-linked dextran
polymeric

Groupe de la matrice active

dextran-epichlorohydrin phase

Taille des particules

17-132 μm (dry)

cleaning

2-13

working range

2-13

Technique de séparation

size exclusion (SEC)

Description générale

HiPrep™ 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

Caractéristiques et avantages

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

Adéquation

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Informations légales

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Articles

Automated Purification of Antibodies Using ÄKTA Chromatography Systems

This page describes automated purification of antibodies using ÄKTA chromatography systems.

General Considerations for Purification of GST-tagged Protein

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

Performing a purification and on-column refolding of an insoluble histidine-tagged protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

Afficher tout

Protocoles

Affinity Chromatography in a Purification Strategy (CIPP)

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

General Instructions for Affinity Purification Using HiTrap® Columns

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

Tag Removal by Enzymatic Cleavage

This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

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