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E6632

Sigma-Aldrich

Epoxy-activated-Agarose

lyophilized powder

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About This Item

Numéro MDL:
MFCD00163561
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

Forme

lyophilized powder

Ampleur du marquage

≥20 μmol per mL

Technique(s)

affinity chromatography: suitable

Matrice

cross-linked 4% beaded agarose

Groupe de la matrice active

epoxy

Fixation de matrice

through epoxy to hydroxyl

Espaceur de matrice

12 atoms

Gonflement

1 g swells to ~3 mL

Adéquation

suitable for chromatography

Température de stockage

−20°C

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Description générale

Epoxy-activated-Agarose is a resin. Ligands can be covalently linked to agarose via the interaction of the nucleophilic groups of the ligand with the epoxy group.
when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Application

Epoxy-activated-Agarose is a lyophilized powder, stabilized with lactose, which is used in affinity chromatography, protein chromatography and activated/functionalized matrices. Epoxy-activated agarose has been used in studies informing anti-proliferative activity on human-derived cancer cells as well as cancer prevention.

Autres remarques

Ligand: 1,4-bis(2:3-Epoxypropoxy)butane
Attachment: one epoxy group
Spacer: provides a 12-atom spacer when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Forme physique

Lyophilized powder stabilized with lactose

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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S Ranganathan et al.
Biochimica et biophysica acta, 1182(3), 311-316 (1993-10-20)
Glyoxalase-I (Gly-I) is part of the glyoxalase system which converts methylglyoxal to D-lactic acid via an S-D-lactoylglutathione intermediate. This glutathione (GSH)-binding protein was purified from human colon tumors and corresponding normal tissue. The GSH-affinity purified fraction from normal human colon
G J Fennington et al.
Microbiology (Reading, England), 142 ( Pt 2), 321-330 (1996-02-01)
The Rhizobium leguminosarum bv. trifolii BAL fructokinase (frk) gene was isolated on a 2 center dot 4 kb BamHI fragment from the cosmid pLA72 by complementation analysis of the Tn5-induced frk mutant BAL79, and confirmed by hybridization analysis. The nucleotide
Zhirong Wang et al.
Biochemical and biophysical research communications, 323(3), 743-749 (2004-09-24)
The phytochemical resveratrol (trans-3,4',5-trihydroxystilbene) is a naturally occurring polyphenol with a plethora of health-beneficial properties, including a preventive role in cancer. We surmise that resveratrol may exert its diverse biological effects by interacting with specific target proteins, denoted RTPs. To
J J Malinowski et al.
Protein science : a publication of the Protein Society, 4(10), 2149-2155 (1995-10-01)
Interleukin-1 beta converting enzyme (ICE) is a cysteine protease that catalyzes the conversion of the inactive precursor form of IL-1 beta to an active mature form. The mature form of IL-1 beta is involved in mediating inflammatory responses and in
Adel M Nour et al.
Infection and immunity, 77(3), 1262-1271 (2009-01-07)
Multiple microbial components trigger the formation of an inflammasome complex that contains pathogen-specific nucleotide oligomerization and binding domain (NOD)-like receptors (NLRs), caspase-1, and in some cases the scaffolding protein ASC. The NLR protein Nalp1b has been linked to anthrax lethal
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