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198161

Sigma-Aldrich

Toluidine Blue O

certified by the Biological Stain Commission

Synonyme(s) :

Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride

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About This Item

Formule empirique (notation de Hill):
C15H16ClN3S
Numéro CAS:
92-31-9
Poids moléculaire :
305.83
Numéro C.I. (Colour Index):
52040
Numéro CE :
202-146-2
Numéro MDL:
MFCD00011934
Code UNSPSC :
12171500
ID de substance PubChem :
24851865
Nomenclature NACRES :
NA.47

Qualité

certified by the Biological Stain Commission

Niveau de qualité

200

Forme

powder

Composition

Dye content, 80%

Couleur

dark green

λmax

626 nm

ε (coefficient d'extinction)

≥13000 at 241-247 nm in H2O
≥28000 at 287-293 nm in H2O
≥40000 at 625-635 nm in H2O

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C

InChI

1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H

Clé InChI

GEDVVYWLPUPJJZ-UHFFFAOYSA-N

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Description générale

A metachromatic dye suitable for a wide variety of histological staining procedures.

Application

Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.

Actions biochimiques/physiologiques

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.

Adéquation

Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
MKCK0257
MKCH9602
MKCC2845
MKBW2865V
MKBT8757V

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The adsorption kinetics and removal of cationic dye, Toluidine Blue O, from aqueous solution with Turkish zeolite
Alpat S K
Journal of Hazardous Materials, 151(1), 213-220 (2008)
Factors influencing the susceptibility of Gram-negative bacteria to toluidine blue O-mediated lethal photosensitization
komerik N
Journal of Applied Microbiology, 92(4), 618?623-618?623 (2002)
A TRIBASIC STAIN FOR THIN SECTIONS OF PLASTIC-EMBEDDED, OSO4-FIXED TISSUES.
P M GRIMLEY
Stain technology, 39, 229-233 (1964-07-01)
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There is growing evidence that the quality of spermatozoa decreases with age and that children of older fathers have a higher incidence of birth defects and genetic mutations. The free radical theory of aging proposes that changes with aging are
Polychromatic staining of plant cell walls by toluidine blue O
Brien T P O
Protoplasma, 59(2), 368- 373 (1964)
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