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Key Documents

MABF264

Sigma-Aldrich

Anti-AIP1/WD repeat-containing protein 1, clone 13F10 Antibody

clone 13F10, from rat

Synonyme(s) :

WD repeat-containing protein 1, Actin-interacting protein 1, AIP1, AIP1/WD repeat-containing protein 1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rat

Niveau de qualité

300

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

13F10, monoclonal

Espèces réactives

mouse

Technique(s)

western blot: suitable

Isotype

IgG2bκ

Numéro d'accès NCBI

NP_035845

Numéro d'accès UniProt

O88342

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Wdr1(22388)

Description générale

Actin-interacting protein 1 (AIP1), also known as WD repeat-containing protein 1 (Wdr1), is a key player in cytoskeletal rearrangements and actin mobilization, and thus plays a role in many cellular situations from movement to cytokinesis. AIP1 interacts with Cofilin as well, and Cofilin regulates actin filament dynamics by stimulating actin filament disassembly and also plays a critical role in cytokinesis and chemotactic migration. Knockdown of AIP1 in HeLa cells increased the percentage of multinucleate cells and the mechanics of cytokinesis were altered with an excessive buildup of actin filaments at telophase. The results could be relieved by overexpression of Cofilin pointing to AIP1’s critical role in Cofilin-mediated actin filament disassembly. Furthermore, AIP1 inhibition caused abnormal cell migration in response to cytokine stimulation leading to a non-directional migration phenotype thus indicating that AIP1 plays an important directional role in response to stimuli as well. Finally AIP1, as one might expect, plays a critical role in development of cells and tissues, especially in epithelial morphogenesis for instance.

Immunogène

Recombinant protein corresponding to mouse AIP1/WD repeat-containing protein 1.

Application

This Anti-AIP1/WD repeat-containing protein 1, clone 13F10 Antibody is validated for use in Western Blotting for the detection of AIP1/WD repeat-containing protein 1.
Western Blotting Analysis: A representative lot detected AIP1/WD repeat-containing protein 1 in both endogenous and transfected mouse AIP1 in western blots of cell lysates from J774 cells, 293T cells and 293T cells transfected with HA-tagged mouse Aip1 (Li, J., et al. (2007). Nature. 9(3):276-286).

Qualité

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected AIP1/WD repeat-containing protein 1 in 10 µg of RAW264.7 cell lysate.

Description de la cible

~66 kDa observed

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

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Code de la classe de stockage

10 - Combustible liquids

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Consulter la Bibliothèque de documents

AIP1 acts with cofilin to control actin dynamics during epithelial morphogenesis.
Chu, D; Pan, H; Wan, P; Wu, J; Luo, J; Zhu, H; Chen, J
Development null
Juying Li et al.
Nature cell biology, 9(3), 276-286 (2007-02-13)
Coordinated regulation of cell migration, cytokine maturation and apoptosis is critical in inflammatory responses. Caspases, a family of cysteine proteases, are known to regulate cytokine maturation and apoptosis. Here, we show that caspase-11, a mammalian pro-inflammatory caspase, regulates cell migration
Critical roles of actin-interacting protein 1 in cytokinesis and chemotactic migration of mammalian cells.
Kato, A; Kurita, S; Hayashi, A; Kaji, N; Ohashi, K; Mizuno, K
The Biochemical Journal null

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