MABE1791M

Anti-KAT2A (GCN5) Antibody, clone 2GC-2C11

clone 2GC-2C11, from mouse

• Synonyme(s) : Histone acetyltransferase KAT2A, EC: 2.3.1.48, General control of amino acid synthesis protein 5-like 2, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 2GC-2C11, monoclonal
• Espèces réactives: human, mouse
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_066564
• Numéro d'accès UniProt: Q92830
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KAT2A(2648)

Description

Description générale

Histone acetyltransferase KAT2A (UniProt: Q92830; also known as EC: 2.3.1.48, General control of amino acid synthesis protein 5-like 2, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97) is encoded by the KAT2A (also known as GCN5, GCN5L2, HGCN5) gene (Gene ID: 2648) in human. Histone acetyltransferase KAT2A belongs to the acetyltransferase family and is expressed in all tissues tested. However, its expression is highest in ovaries. It has three acetyl-CoA-binding regions (aa 579-581; 586-592; 617-620) and one bromodomain. It functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. It displays significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. KAT2A is also shown to acetylate non-histone proteins, such as CCAAT/enhancer-binding protein beta (CEBPB). In case of HIV-1 infection, histone acetyltransferase KAT2A is recruited by the viral protein Tat and it can regulates Tat′s transactivating activity and may help in inducing chromatin remodeling of proviral genes. Two isoforms of histone acetyltransferase KAT2A have been reported that are produced by alternative splicing. Isoform 2 lacks the first 410 amino acids.

Spécificité

Clone 2GC-2C11 detects Histone acetyltransferase KAT2A in human and murine cells. It targets an epitope within 15 amino acids from the N-terminal region.

Immunogène

Ovalbumin-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Histone acetyltransferase KAT2A.

Application

Anti-KAT2A (GCN5), clone 2GC-2C11, Cat. No. MABE1791, is a highly specific mouse monoclonal antibody that targets Histone acetyltransferase KAT2A and has been tested in Western Blotting and Immunoprecipitation.

Western Blotting Analysis: 2 µg/mL from a representative lot detected KAT2A (GCN5) in 10 µg of NIH/3T3 cell lysate.

Immunoprecipitation Analysis: A representative lot detected KAT2A (GCN5) in Immunoprecipitation applications (Brand, M., et. al. (2001). EMBO J. 20(12):3187-96).

Western Blotting Analysis: A representative lot detected KAT2A (GCN5) in Western Blotting applications (Riss, A., et. al. (2015). J Biol Chem. 290(48):28997-9009; Helmlinger, D., et. al. (2006). PLoS Biol. 4(3):e67; Brand, M., et. al. (2001). EMBO J. 20(12):3187-96).

Qualité

Evaluated by Western Blotting in HeLa nuclear extracts.

Western Blotting Analysis: 2 µg/mL of this antibody detected KAT2A (GCN5) in 10 µg of HeLa cells nuclear extract.

Description de la cible

~94 kDa observed; 93.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable