MABE1790

Anti-ADAR1 Antibody, clone RD4B11

clone RD4B11, from rat

• Synonyme(s) : Double-stranded RNA-specific adenosine deaminase, EC: 3.5.4.37, DRADA, RNA adenosine deaminase 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rat
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: RD4B11, monoclonal
• Espèces réactives: mouse, human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_062629.3
• Numéro d'accès UniProt: Q99MU3
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Adar(56417)

Description

Description générale

Double-stranded RNA-specific adenosine deaminase (UniProt: Q99MU3; also known as EC: 3.5.4.37, DRADA, RNA adenosine deaminase 1) is encoded by the Adar gene (Gene ID: 56417) in murine species. ADAR catalyzes the editing of adenosine-to-inosine (A to I) by deaminating genomically encoded A-to-I in double stranded RNA (DsRNA). A-to-I editing predominantly occurs in noncoding, repetitive elements and short interspersed nuclear elements (SINEs). A-to-I editing affects gene expression and function in several ways, including mRNA translation by changing codons and hence the amino acid sequence of proteins. It also affects pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; and genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication. In mammals three different ADAR proteins have been described: ADAR1, -2, and -3. ADAR1 is shown to be expressed during embryonic and postnatal development and is present predominantly in the nucleus. Highest levels of ADAR1 are reported in brain and spleen and lowest levels are observed in liver. Five different isoforms of ADAR1 have been reported that are produced by alternative splicing. Isoforms 1 and 2 (long forms) are predominantly found in cytoplasm and shuttle between the cytoplasm and nucleus. Isoforms 3 and 4 (short forms starting at Met-519) are exclusively found in the nucleolus. Under normal conditions long forms are dominant, however, under conditions of inflammation short forms are selectively induced. (Ref.: Liddicoat, BJ et al., (2015). Science 349 (6252); 1115-1120).

Spécificité

Clone RD4B11 is a rat monoclonal antibody that detects Double-stranded RNA-specific adenosine deaminase in human and murine cells.

Immunogène

His-tagged recombinant fragment corresponding to 401 amino acids from the internal region of murine Double-stranded RNA-specific adenosine deaminase (ADAR1). Immunogen sequnece is conserved isoforms 1 and 2.

Application

Anti-ADAR1, clone RD4B11, Cat. No. MABE1790, is a highly specific rat monoclonal antibody that targets ADAR1 and has been tested for use in Western Blotting.

Western Blotting Analysis: A representative lot detected ADAR1 in Western Blotting applications (Liddicoat, B.J., et. al. (2015). Science. 349(6252):1115-20).

Qualité

Evaluated by Western Blotting in Kusa4b10 mouse cell line overexpressing mouse ADAR1 p110.

Western Blotting Analysis: 4 µg/mL of this antibody detected ADAR1 in Kusa4b10 mouse cell line overexpressing mouse ADAR1 p110.

Description de la cible

~110 kDa observed; 130.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.