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535142

Millipore

Protease Inhibitor Cocktail Set I, Animal-Free

The Protease Inhibitor Cocktail Set I, Animal-Free controls the activity of Protease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

Synonyme(s) :

Animal-Free Protease Inhibitor Mix, Protease inhibitor

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About This Item

Code UNSPSC :
12352200

Forme

lyophilized

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
desiccated (hygroscopic)

Conditions d'expédition

wet ice

Température de stockage

−20°C

Catégories apparentées

Description générale

A cocktail of five protease inhibitors for the inhibition of a broad range of proteases and esterases. Each vial, when reconstituted with 1 ml H2O, yields a 100X stock solution. When diluted to 1X the cocktail contains 500 µM AEBSF, HCl (Cat. No. 101500), 150 nM Aprotinin (Cat. No. 616371), 1 µM E-64 (Cat. No. 324890), 0.5 mM EDTA, Disodium Salt and 1 µM Leupeptin Hemisulfate (Cat. No. 108975). Available as 1 ml or as a set of 10 x 1 ml.

Application

Protease Inhibitor Cocktail Set I, Animal-Free has been used:
  • as a supplement in cell lysis buffer to extract proteins from non-small-cell lung carcinoma (NSCLC) cell lines
  • in NP-40 buffer to lyse the cells for sodium dodecyl sulfate-12% polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting
  • as a component in lysis buffer to lyse transfected Huh7 cells and to wash the pellets to detect hepatitis B virus (HBV) C protein phosphorylation in the cell

Actions biochimiques/physiologiques

Protease inhibitor cocktails and specific protease inhibitors are valuable resources for scientists involved in proteomic studies, such as protein expression, characterization, biomarker discovery, post-translational modification mapping, protein profiling, and quantitative protein measurement. By adding a protease inhibitor cocktail or specific inhibitor, the integrity of proteins is preserved for subsequent analysis and further sample characterization.

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial in 1 ml H₂O to yield a 100X stock solution.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

CorrosionExclamation markHealth hazard
GHS05,GHS07,GHS08

Mention d'avertissement

Danger

Mentions de danger

H314 - H332 - H373

Classification des risques

Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation

Organes cibles

Respiratory Tract

Code de la classe de stockage

8A - Combustible, corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

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Jumi Kim et al.
Microorganisms, 10(3) (2022-03-27)
Ca2+/calmodulin-dependent protein kinase II (CaMKII), which is involved in the calcium signaling pathway, is an important regulator of cancer cell proliferation, motility, growth, and metastasis. The effects of CaMKII on hepatitis B virus (HBV) replication have never been evaluated. Here

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